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Cellular cryo-electron tomography has changed the way biological systems can be analyzed and modelled. Using this approach, intact cells can now be imaged in their native state and at high resolution, allowing both the quantitative analysis of cellular constituents, as well as modelling of dynamic cellular processes. Some of these might be rare or localized to specific sites within a cell.
In this webcast, the speaker will describe use of a 3D-correlative workflow, combining confocal cryo-fluorescence microscopy, focused ion beam milling, zero-loss energy filtering, and cryo-electron tomography to study rare biological events.
Join this high-resolution journey from the membrane to the center of the cell to learn: